NGS Learning Zone

The best place to learn about Next Generation Sequencing. At YouSeq, we are aware that Next Generation Sequencing is a large, complex subject. We’re determined to simplify it.

We built the NGS learning zone as a place with high quality, easy to understand material to help you understand the subject.

So where do you want to start?

New to NGS

The basics of Next Generation Sequencing.

A Beginners guide to Next Generation Sequencing (NGS)

The basics of NGS explained quickly and clearly

Adapters and indexes

A simple explanation of what adapters are and what they do in NGS

Bioinformatics and Data Analysis

A simple look at the world of data analysis and Bioinformatics

Whole genome, Exome or targeted sequencing

A quick and easy explanation of the main categories and applications of Next Generation Sequencing

NGS Library Prep methods

Why are there so many different NGS Library prep methods?

Bit of an expert

Learn more about NGS with some more detail.

Illumina Sequencing by Synthesis (SBS) explained

This video, produced by Illumina, gives an excellent animated explanation of how the Illumina SBS technology works.

Ion Torrent™ next-gen sequencing technology

Next-generation sequencing (NGS) utilizes massively parallel sequencing to generate thousands of megabases of sequence information per day...

Genomics and Personalised Medicine

The 100,000 Genomes Project is transforming the NHS and enabling personalised medicine for patients, now and in the future.

New study challenges standard for validating DNA sequencing results

A new study by the National Human Genome Research Institute (NHGRI) is challenging traditional practices for validating DNA sequencing results.

Your questions answered

A collection of DNA or cDNA fragments prepared for sequencing by a performing a series of enzymatic steps. These steps are commonly referred to as the Library Preparation.
- Single-End Read (SE), which provides sequence from only one end of the DNA insert - Paired-End Read (PE), which provides sequence from both ends of a DNA insert - Mate Pair Read (ME), Similar to paired-end but both reads come from a single strand of DNA
The number of times a nucleotide is sequenced. The deeper the read depth, the higher degrees of confidence in the base calls.
Yes you can. The MasterMix is designed to be robust and perform well with any good quality NGS panels.
Yes, YouSeq custom panels can be used on all illumina platforms.
Unlike our competitors YouSeq custom panels are developed by our expert scientists and are not algorithm driven. All our panels are optimised for guaranteed performance delivering high quality sequencing data. YouSeq custom panels also come with all the reagents you need to prepare your library, it’s a complete solution. 
Due to the targeting regions being amplified using PCR, YouSeq’s kits work with as little as 5 ng of purified genomic DNA.
PhiX is a ready-made DNA library. It’s useful as a positive control to check all is working well with your sequencing run. It also adds “complexity” which is useful. DNA sequencers don’t like to sequence multiple identical libraries at the same time so adding PhiX mixes things up a bit and helps your sequencer perform better.
The ability to insert the correct base during a PCR cycle. Therefore, having a high fidelity master mix would mean having a low error base incorporation rate and higher quality libraries
The number of bases sequenced by your sequencer as a continuous length.

Any questions?

We love to help people out. Get in touch with any of your questions.

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What's new?

Just a few of the latest products we’re proud of...

YouSeq High-Fidelity 2X Master Mix

Power through long transcripts, with high GC content and ultra low error rates

NGS Library Quantification Kit

Simple to use kit for checking your NGS library quantity and quality before you sequence it

NGS Library Bead Clean-up Kit

Fast and easy removal or PCR artefacts and contaminants in NGS Libraries

YouSeq NGS Multiplex DNA Mastermix

A high performance MasterMix built especially for Amplicon based NGS Library prep