THE ONE 16S NGS LIBRARY PREP KIT

The World's fastest and easiest 16S NGS Library prep kit

The ONE 16S NGS Library Preparation Kit, including a Library Quantification kit provides a simple and comprehensive NGS workflow, designed to take you from sample to analysis report with minimal hands-on time. It contains all of the reagents necessary to create a ready-to-sequence NGS library in minutes. In a breakthrough kit format, the user simply performs one closed tube qPCR reaction. The variable regions V3/4 are targeted, amplified and adapters added in a single reaction. The quantitative PCR read out simultaneously quantifies each library so they can be pooled precisely. Then a simple bead-clean completes the workflow.

After sequencing, the data can then be loaded on to our cloud solution for rapid analysis. A detailed report is typically returned within 15 minutes.

96 samples (Illumina)

Cat no. YS-NGS-ONE16SLQ-IL-961

96 samples (Illumina)

Cat no. YS-NGS-ONE16SLQ-IL-96

For Research Use Only. Not intended for diagnostic use.

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Data analysis is included in the kit price. Load fastq files to our cloud solution and receive a detailed interactive report back in around 15 minutes.
Reagents for Library preparation, quantification and bead clean up of 96 samples

Oligo mix for each sample
The ONE qPCR Mastermix

Magnetic beads
Wash buffer
Elution buffer

Library specific qPCR primers
qPCR MasterMix premixed with SYBRgreen
4 x positive control templates to create standard curve
NGS library dilution buffer

Full automated data analysis is included in the price of your kit

Kits are available for use on Illumina sequencing platforms.

Perfect 16S Libraries are created in one simple closed-tube reaction

What is 16S sequencing? And why is it so important?

Your Questions Answered

A collection of DNA or cDNA fragments prepared for sequencing by a performing a series of enzymatic steps. These steps are commonly referred to as the Library Preparation.
The number of times a nucleotide is sequenced. The deeper the read depth, the higher degrees of confidence in the base calls.

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