The ONE 16S NGS kit

The World's fastest and easiest 16S NGS Library prep kit

Create a ready to sequence 16S Library in one closed tube reaction. The ONE 16S NGS kit contains all of the reagents necessary to create a ready-to-sequence NGS library in minutes. In a breakthrough kit format, the user simply performs one closed tube qPCR reaction. The variable regions V3/4 are targeted, amplified and adapters are added in a single reaction. The quantitative PCR read out simultaneously quantifies each library so they can be pooled precisely. Then a simple bead-clean completes the workflow.
After sequencing, the data can then be loaded on to our cloud for rapid analysis. A detailed report is typically returned within 15 minutes.

Data Analysis

Data analysis is included in the kit price. Load fastq files to our cloud solution and receive a detailed interactive report back in around 15 minutes.

Kit contents

Reagents for Library preparation and bead clean up of 8, 48 or 96 samples

Oligo mix for each sample
The ONE qPCR Mastermix
Magnetic beads
Wash buffer
Elution buffer

Full automated data analysis is included in the price of your kit

Technical specification

Kits are available for use on Illumina and Ion Torrent sequencing platforms.

Perfect 16S Libraries are created in one simple closed-tube reaction

Downloads

The ONE index sheet 8 set A

The ONE index sheet 96 set A