NGS Library Quantification Kit

Simple to use kit for checking your NGS library quantity and quality before you sequence it

The YouSeq NGS Library quantification kit employs qPCR as a highly specific and. Sensitive method to calculate the amount of DNA library present with successfully incorporated adapters. Results are compared to a set of standards for precise quantification.

Dilution buffer is also supplied to dilute your DNA library accordingly, ready to be sequenced.

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Cat no. YS-LQC-96

Kit contents

Library specific qPCR primers,
qPCR Mastermix premixed with SYBRgreen,
4 x positive control templates to create standard curve,
NGS library dilution buffer

Technical specification

qPCR is the most accurate method for quantification of an NGS Library. It is also far more cost effective and requires less bench time, especially in higher throughput laboratories vs Bioanalyzer of Spectrophotometer

Your Questions Answered

What does a 'library' mean?

A collection of DNA or cDNA fragments prepared for sequencing by a performing a series of enzymatic steps. These steps are commonly referred to as the Library Preparation.

What is the minimum sample DNA that I can use?

Due to the targeting regions being amplified using PCR, YouSeq’s kits work with as little as 5 ng of purified genomic DNA.