16S NGS Library Preparation Kit

A quick and easy NGS Library prep kit for 16S rRNA sequencing

16S rRNA sequencing is a groundbreaking method for identifying bacterial species using NGS. The YouSeq 16S kit is simple and easy to use and includes all reagents necessary to produce a high-quality, NGS-ready, library. The Library is then sequenced and an automatically generated report identifies the bacteria present in the microbiome of the sample.

Cat no. YS-16S-IL-96

Pack size:

We currently recommend the use of Illumina BaseSpace 16S app for analysis.
Target specific primers,
NGS MasterMix,
Adapters,
Bead clean up kit

Other items to be supplied by the user:
PCR instrument,
Illumina NGS sequencer,
Sequencing reagents,
Magnetic rack

16S Metagenomic Sequencing Library Preparation Kit

Metagenomics is the study of genetic material recovered directly from environmental samples. This can include clinical samples taken from wounds, swabs, blood, urine etc. While traditional microbiology, antibody-based methods or qPCR can only define if a particular species is present or not; Metagenomics by NGS has the power to produce a precise profile of microbial diversity in a given sample. This gives clinicians and researchers alike, unprecedented power to understand the complex nature of a bacterial infection.
16S Primers are designed to target the Bacterial V3 and V4 regions.

Bioinformatic analysis is recommended using Illumina BaseSpace 16S Module.

Sold for Research Use Only

Your Questions Answered

Yes, YouSeq custom panels can be used on all illumina platforms.
Due to the targeting regions being amplified using PCR, YouSeq’s kits work with as little as 5 ng of purified genomic DNA.
PhiX is a ready-made DNA library. It’s useful as a positive control to check all is working well with your sequencing run. It also adds “complexity” which is useful. DNA sequencers don’t like to sequence multiple identical libraries at the same time so adding PhiX mixes things up a bit and helps your sequencer perform better.

More Products

Human gDNA quantification kit

Fast and accurate quantification of gDNA template by qPCR

YouSeq High-Fidelity 2X Master Mix

Power through long transcripts, with high GC content and ultra low error rates

NGS Library Quantification Kit

Simple to use kit for checking your NGS library quantity and quality before you sequence it

NGS Library Bead Clean-up Kit

Fast and easy removal or PCR artefacts and contaminants in NGS Libraries

Related Features

Download our free beginner's guide to Next Generation Sequencing

A simple, easy to read look at the basics of NGS

BBC Article - What does personalised medicine mean for you?

BBC Article describing the link between DNA sequence and personalised medicine.

NGS Library Prep methods

Why are there so many different NGS Library prep methods?

Genomics and Personalised Medicine

The 100,000 Genomes Project is transforming the NHS and enabling personalised medicine for patients, now and in the future.